Fig. 1
From: A safety type of genetically engineered bacterium that degrades chemical pesticides
Construction map of the recombinant plasmid. Construction map of the plasmid pL-EGFP-B1. The egfp gene without the stop codon was cloned into the plasmid pBV220 to construct the plasmid pBV-EGFP. The entire sequence of pBV-EGFP except the cIts857 gene was amplified by PCR and self-circularized to generate the plasmid pL-EGFP. Then, the B1 gene was inserted to generate the final plasmid pL-EGFP-B1. PR and PL indicate the lambda PR promoter and PL promoter, respectively. MCS is the multiple cloning sites and ori is the origin