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Table 1 Primers used for PCR amplification

From: Serotype distribution, antimicrobial susceptibility, antimicrobial resistance genes and virulence genes of Salmonella isolated from a pig slaughterhouse in Yangzhou, China

Target genes

Nucleotide sequences

Size (bp)

Salmonella spp. detection primer sets

 stn-F

5′-CTTTGGTCGTAAAATAAGGCG-3′

260

 stn-R

5′-TGCCCAAAGCAGAGAGATTC-3′

 hut-F

5′-ACTGGCGTTATCCCTTTCTCTGCTG-3′

495

 hut-R

5′-ATGTTGTCCTGCCCCTGGTAAGAGA-3′

16S rRNA primer set

 27F

5′-AGAGTTTGATCCTGGCTCAG-3′

1466

 1492R

5′-TACGGTTACCTTGTTACGACTT-3′

Antimicrobial resistance gene primer sets

 

 Quinolones

  qnrA-F

5′-TTCAGCAAGAGGATTTCTCA-3′

500

  qnrA-R

5′-GGCAGCACTATTACTCCCAA -3′

  qnrB-F

5′-CCTGAGCGGCACTGAATTTT-3′

617

  qnrB-R

5′-GTTTGCTGCTCGCCAGTCGA-3′

  qnrC-F

5′-GGGTTGTACATTTATTGAATC-3′

447

  qnrC-R

5′-TCCACTTTACGAGGTTCT-3′

  qnrD-F

5′-TTACGGGGAATAGAGTTA-3′

468

  qnrD-R

5′-AATCAGCCAAAGACCAAT-3′

  qnrS-F

5′-ACATAAAGACTTAAGTGATC-3′

619

  qnrS-R

5′-CAATTAGTCAGGATAAAC-3′

  qepA-F

5′-CCAGCTCGGCAACTTGATAC-3′

570

  qepA-R

5′-ATGCTCGCCTTCCAGAAAA-3′

  oqxA-F

5′-CTCGGCGCGATGATGCTC-3′

392

  oqxA-R

5′-CACTCTTCACGGGAGACGA-3′

  oqxB-F

5′-TTCTCCCCCGGGGGGAAGTCCTCGGC-3′

512

  oqxB-R

5′-CATTTTGGCGCGTA-3′

 Aminoglycosides

  aac(6′)-Ib-F

5′-TTGCGATGCTCTATGAGTGGCTA-3′

482

  aac(6′)-Ib-R

5′-CTCGAATGCCTGGCGTGTT-3′

  aadA1-F

5′-GCGCCATCTCGAACCGACGTT-3′

573

  aadA1-R

5′-GCCCAGTCGGCAGCGACATC-3′

 Sulfonamides

  sul1-F

5′-TGGCGTCGCGACTGCGAAAT-3′

813

  sul1-R

5′-TGGTGACGGTGTTCGGCATTCT-3′

  sul2-F

5′-GTTTCTCCGATGGAGGCCGGT-3′

517

  sul2-R

5′-AGCGAGGTTTCGGGAGCAGC-3′

 Trimethoprim

  dfrA1-F

5′-AGTGCCAAAGGTGAACAGCTCCT-3′

308

  dfrA1-R

5′-ACATCACCTTCCGGCTCGATGTCT-3′

 β-lactamase

  blaOXA-1-F

5′-ATGAAAAACACAATACATATC-3′

830

  blaOXA-1-R

5′-AATTTAGTGTGTTTAGAATGG-3′

  blaPSE-1-F

5′-CGCTTCCCGTTAACAAGTAC-3′

420

  blaPSE-1-R

5′-CTGGTTCATTTCAGATAGCG-3′

  blaTEM-F

5′-ATAAAATTCTTGAAGACGAAA-3′

1080

  blaTEM-R

5′-GACAGTTACCAATGCTTAATC-3′

  BlaCMY-2-F

5′-TGGCGGTTGCCGTTATCTAC-3′

210

  BlaCMY-2-R

5′-CCCGTTTTATGCACCCATGA-3′

 Tetracyclines

  tetA-F

5′-TGGTCCGGAGGCCAGACGTG-3′

867

  tetA-R

5′-TTCCGAGCATGAGTGCCCGC-3′

  tetB-F

5′-GGAGCTACTGGGGCTGTCGCACC-3′

374

  tetB-R

5′-ACCCACACCGTTGCGGGAAT-3′

  tetG-F

5′-TCTTGCAGGAGCCGCAGTCGAT-3′

721

  tetG-R

5′-GGCCGGCATGCCAACACCC-3′

 Chloramphenicols

  catA1-F

5′-TCTTGCCCGCCTGATGAATGC-3′

388

  catA1-R

5′-AACCTGAATCGCCAGCGGCA-3′

  floR-F

5′-AACCCGCCCTCTGGATCAAGTCAA-3′

549

  floR-R

5′-CAAATCACGGGCCACGCTGTATC-3′

Virulence gene primer sets

  mogA-F

5′-ATTGGCTTAGTTTCTATCTCCG-3′

419

  mogA-R

5′-CCTTCCAGCGTTTCTTTGA-3′

  pvB-F

5′-CCGTAGAGCAGACGCTGTAAGC-3′

1856

  spvB-R

5′-GTATCTATGAGTTGAGTACCCCTATG-3′

  spvC-F

5′-CCGCAAAGTAGTGCATCTAAAC-3′

919

 spvC-R

5′-CCATACTTACTCTGTCATCAAACG-3′