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AMB Express

Table 1 Primers used for PCR amplification

From: Serotype distribution, antimicrobial susceptibility, antimicrobial resistance genes and virulence genes of Salmonella isolated from a pig slaughterhouse in Yangzhou, China

Target genes Nucleotide sequences Size (bp)
Salmonella spp. detection primer sets
 stn-F 5′-CTTTGGTCGTAAAATAAGGCG-3′ 260
 stn-R 5′-TGCCCAAAGCAGAGAGATTC-3′
 hut-F 5′-ACTGGCGTTATCCCTTTCTCTGCTG-3′ 495
 hut-R 5′-ATGTTGTCCTGCCCCTGGTAAGAGA-3′
16S rRNA primer set
 27F 5′-AGAGTTTGATCCTGGCTCAG-3′ 1466
 1492R 5′-TACGGTTACCTTGTTACGACTT-3′
Antimicrobial resistance gene primer sets  
 Quinolones
  qnrA-F 5′-TTCAGCAAGAGGATTTCTCA-3′ 500
  qnrA-R 5′-GGCAGCACTATTACTCCCAA -3′
  qnrB-F 5′-CCTGAGCGGCACTGAATTTT-3′ 617
  qnrB-R 5′-GTTTGCTGCTCGCCAGTCGA-3′
  qnrC-F 5′-GGGTTGTACATTTATTGAATC-3′ 447
  qnrC-R 5′-TCCACTTTACGAGGTTCT-3′
  qnrD-F 5′-TTACGGGGAATAGAGTTA-3′ 468
  qnrD-R 5′-AATCAGCCAAAGACCAAT-3′
  qnrS-F 5′-ACATAAAGACTTAAGTGATC-3′ 619
  qnrS-R 5′-CAATTAGTCAGGATAAAC-3′
  qepA-F 5′-CCAGCTCGGCAACTTGATAC-3′ 570
  qepA-R 5′-ATGCTCGCCTTCCAGAAAA-3′
  oqxA-F 5′-CTCGGCGCGATGATGCTC-3′ 392
  oqxA-R 5′-CACTCTTCACGGGAGACGA-3′
  oqxB-F 5′-TTCTCCCCCGGGGGGAAGTCCTCGGC-3′ 512
  oqxB-R 5′-CATTTTGGCGCGTA-3′
 Aminoglycosides
  aac(6′)-Ib-F 5′-TTGCGATGCTCTATGAGTGGCTA-3′ 482
  aac(6′)-Ib-R 5′-CTCGAATGCCTGGCGTGTT-3′
  aadA1-F 5′-GCGCCATCTCGAACCGACGTT-3′ 573
  aadA1-R 5′-GCCCAGTCGGCAGCGACATC-3′
 Sulfonamides
  sul1-F 5′-TGGCGTCGCGACTGCGAAAT-3′ 813
  sul1-R 5′-TGGTGACGGTGTTCGGCATTCT-3′
  sul2-F 5′-GTTTCTCCGATGGAGGCCGGT-3′ 517
  sul2-R 5′-AGCGAGGTTTCGGGAGCAGC-3′
 Trimethoprim
  dfrA1-F 5′-AGTGCCAAAGGTGAACAGCTCCT-3′ 308
  dfrA1-R 5′-ACATCACCTTCCGGCTCGATGTCT-3′
 β-lactamase
  blaOXA-1-F 5′-ATGAAAAACACAATACATATC-3′ 830
  blaOXA-1-R 5′-AATTTAGTGTGTTTAGAATGG-3′
  blaPSE-1-F 5′-CGCTTCCCGTTAACAAGTAC-3′ 420
  blaPSE-1-R 5′-CTGGTTCATTTCAGATAGCG-3′
  blaTEM-F 5′-ATAAAATTCTTGAAGACGAAA-3′ 1080
  blaTEM-R 5′-GACAGTTACCAATGCTTAATC-3′
  BlaCMY-2-F 5′-TGGCGGTTGCCGTTATCTAC-3′ 210
  BlaCMY-2-R 5′-CCCGTTTTATGCACCCATGA-3′
 Tetracyclines
  tetA-F 5′-TGGTCCGGAGGCCAGACGTG-3′ 867
  tetA-R 5′-TTCCGAGCATGAGTGCCCGC-3′
  tetB-F 5′-GGAGCTACTGGGGCTGTCGCACC-3′ 374
  tetB-R 5′-ACCCACACCGTTGCGGGAAT-3′
  tetG-F 5′-TCTTGCAGGAGCCGCAGTCGAT-3′ 721
  tetG-R 5′-GGCCGGCATGCCAACACCC-3′
 Chloramphenicols
  catA1-F 5′-TCTTGCCCGCCTGATGAATGC-3′ 388
  catA1-R 5′-AACCTGAATCGCCAGCGGCA-3′
  floR-F 5′-AACCCGCCCTCTGGATCAAGTCAA-3′ 549
  floR-R 5′-CAAATCACGGGCCACGCTGTATC-3′
Virulence gene primer sets
  mogA-F 5′-ATTGGCTTAGTTTCTATCTCCG-3′ 419
  mogA-R 5′-CCTTCCAGCGTTTCTTTGA-3′
  pvB-F 5′-CCGTAGAGCAGACGCTGTAAGC-3′ 1856
  spvB-R 5′-GTATCTATGAGTTGAGTACCCCTATG-3′
  spvC-F 5′-CCGCAAAGTAGTGCATCTAAAC-3′ 919
 spvC-R 5′-CCATACTTACTCTGTCATCAAACG-3′
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