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Table 1 Primers used for PCR amplification

From: Serotype distribution, antimicrobial susceptibility, antimicrobial resistance genes and virulence genes of Salmonella isolated from a pig slaughterhouse in Yangzhou, China

Target genesNucleotide sequencesSize (bp)
Salmonella spp. detection primer sets
 stn-F5′-CTTTGGTCGTAAAATAAGGCG-3′260
 stn-R5′-TGCCCAAAGCAGAGAGATTC-3′
 hut-F5′-ACTGGCGTTATCCCTTTCTCTGCTG-3′495
 hut-R5′-ATGTTGTCCTGCCCCTGGTAAGAGA-3′
16S rRNA primer set
 27F5′-AGAGTTTGATCCTGGCTCAG-3′1466
 1492R5′-TACGGTTACCTTGTTACGACTT-3′
Antimicrobial resistance gene primer sets 
 Quinolones
  qnrA-F5′-TTCAGCAAGAGGATTTCTCA-3′500
  qnrA-R5′-GGCAGCACTATTACTCCCAA -3′
  qnrB-F5′-CCTGAGCGGCACTGAATTTT-3′617
  qnrB-R5′-GTTTGCTGCTCGCCAGTCGA-3′
  qnrC-F5′-GGGTTGTACATTTATTGAATC-3′447
  qnrC-R5′-TCCACTTTACGAGGTTCT-3′
  qnrD-F5′-TTACGGGGAATAGAGTTA-3′468
  qnrD-R5′-AATCAGCCAAAGACCAAT-3′
  qnrS-F5′-ACATAAAGACTTAAGTGATC-3′619
  qnrS-R5′-CAATTAGTCAGGATAAAC-3′
  qepA-F5′-CCAGCTCGGCAACTTGATAC-3′570
  qepA-R5′-ATGCTCGCCTTCCAGAAAA-3′
  oqxA-F5′-CTCGGCGCGATGATGCTC-3′392
  oqxA-R5′-CACTCTTCACGGGAGACGA-3′
  oqxB-F5′-TTCTCCCCCGGGGGGAAGTCCTCGGC-3′512
  oqxB-R5′-CATTTTGGCGCGTA-3′
 Aminoglycosides
  aac(6′)-Ib-F5′-TTGCGATGCTCTATGAGTGGCTA-3′482
  aac(6′)-Ib-R5′-CTCGAATGCCTGGCGTGTT-3′
  aadA1-F5′-GCGCCATCTCGAACCGACGTT-3′573
  aadA1-R5′-GCCCAGTCGGCAGCGACATC-3′
 Sulfonamides
  sul1-F5′-TGGCGTCGCGACTGCGAAAT-3′813
  sul1-R5′-TGGTGACGGTGTTCGGCATTCT-3′
  sul2-F5′-GTTTCTCCGATGGAGGCCGGT-3′517
  sul2-R5′-AGCGAGGTTTCGGGAGCAGC-3′
 Trimethoprim
  dfrA1-F5′-AGTGCCAAAGGTGAACAGCTCCT-3′308
  dfrA1-R5′-ACATCACCTTCCGGCTCGATGTCT-3′
 β-lactamase
  blaOXA-1-F5′-ATGAAAAACACAATACATATC-3′830
  blaOXA-1-R5′-AATTTAGTGTGTTTAGAATGG-3′
  blaPSE-1-F5′-CGCTTCCCGTTAACAAGTAC-3′420
  blaPSE-1-R5′-CTGGTTCATTTCAGATAGCG-3′
  blaTEM-F5′-ATAAAATTCTTGAAGACGAAA-3′1080
  blaTEM-R5′-GACAGTTACCAATGCTTAATC-3′
  BlaCMY-2-F5′-TGGCGGTTGCCGTTATCTAC-3′210
  BlaCMY-2-R5′-CCCGTTTTATGCACCCATGA-3′
 Tetracyclines
  tetA-F5′-TGGTCCGGAGGCCAGACGTG-3′867
  tetA-R5′-TTCCGAGCATGAGTGCCCGC-3′
  tetB-F5′-GGAGCTACTGGGGCTGTCGCACC-3′374
  tetB-R5′-ACCCACACCGTTGCGGGAAT-3′
  tetG-F5′-TCTTGCAGGAGCCGCAGTCGAT-3′721
  tetG-R5′-GGCCGGCATGCCAACACCC-3′
 Chloramphenicols
  catA1-F5′-TCTTGCCCGCCTGATGAATGC-3′388
  catA1-R5′-AACCTGAATCGCCAGCGGCA-3′
  floR-F5′-AACCCGCCCTCTGGATCAAGTCAA-3′549
  floR-R5′-CAAATCACGGGCCACGCTGTATC-3′
Virulence gene primer sets
  mogA-F5′-ATTGGCTTAGTTTCTATCTCCG-3′419
  mogA-R5′-CCTTCCAGCGTTTCTTTGA-3′
  pvB-F5′-CCGTAGAGCAGACGCTGTAAGC-3′1856
  spvB-R5′-GTATCTATGAGTTGAGTACCCCTATG-3′
  spvC-F5′-CCGCAAAGTAGTGCATCTAAAC-3′919
 spvC-R5′-CCATACTTACTCTGTCATCAAACG-3′