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AMB Express

Table 2 Primers used in this study

From: Development of loop-mediated isothermal amplification coupled with nanoparticle-based lateral flow biosensor assay for Mycoplasma pneumoniae detection

Assay

Primers

Sequence (5′–3′)

Gene

 

F3

GAGCGCTTTAACCAGAAGTT

P1

 

B3

AGGCGCGGTTATATCATCC

 

FIP*

5′-FITC-TGGAGAAACGGGAAAGCGTGGAACGGTAGCTCCTACCCAA-3′

LAMP-LFB

BIP

GGTGCTCGACCAGGTGTTGG-CGGTGGTTATTGCCATACC

 

LF*

5′-Biotin-GAGAAGTGGGATCAGTTTGT-3′

 

LB

TGGATTGGGAATGGGTACA

  1. FITC, fluorescein isothiocyanate; FIP*, 5′-labeled with FITC; LF*, 5′-labeled with biotin
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