Table 1 Oligonucleotide primers sequences encoding for amplification of virulence genes and QACs resistance genes
Primer | Target gene | Primer sequence (5′–3′) | Product (bp) | References |
|---|---|---|---|---|
iss-1 | Iss | F-ATGTTATTTTCTGCCGCTCTG | 266 | Yaguchi et al. (2007) |
iss-2 | R-CTATTGTGAGCAATATACCC | |||
eaeA-1 (intimin) | eaeA | F-ATG CTT AGT GCT GGT TTA GG | 248 | Bisi-Johnson et al. (2011) |
eaeA-2 | R-GCC TTC ATC ATT TCG CTT TC | |||
papC-1 | papC | F-TGATATCACGCAGTCAGTAGC | 501 | Jin et al. (2008) |
papC-2 | R-CCGGCCATATTCACATAA | |||
CFAI-1 | cfaI | F-GCTCTGACCACAATGTTGA | 364 | Ghosal et al. (2007) |
CFAI-2 | R-TTACACCGGATGCAGAATA | |||
QacEΔ1-1 | qacEΔ1 | F-TAA CCCTACACAAATTGGGAGATAT | 362 | Chuanchuen et al. (2007) |
QacEΔ1-2 | R-GCC TCC GCA GCG ACT TCC ACG | |||
QacA/B-1 | qacA/B | F-GCAGAAAGTGCAGAGTTCG | 361 | Noguchi et al. (2005) |
QacA/B-2 | R-CCAGTCCAATCATGCCTG | |||
QacC/D-1 | qacC/D | F-GCCATAAGTACTGAAGTTATTGGA | 195 | |
QacC/D-2 | R-GACTACGGTTGTTAAGACTAAACCT |