Component | Volume (25 µL in all) |
---|---|
10× buffer | 2.5 |
Ex Taq (8000 U/mL) | 0.125 |
dNTP (2.5 mM each) | 2.5 |
Mg2+ (25 mM) | 2.0 |
phoA-P1/P2 (20 µM) | 0.5 |
KMT1-P1/P2 (20 µM) | 0.25 |
ureR-P1/P2 (20 µM) | 0.5 |
toxA-P1/P2 (20 µM) | 0.25 |
invA-P1/P2 (20 µM) | 0.5 |
nuc-P1/P2 (20 µM) | 1.0 |
Betaine (5 M) | 2.0 |
Template DNA (120 ng, each) | 1.0 |
DDH2O | 11.875 |