Fig. 2
Analysis of enzyme-complex formation by NativePAGE™. Electrophoretic mobility of components and assembled complexes on nondenaturing gels. Recombinant S1 (rS1), native S1 (nS1), rXyn11A and rXynXL (Xyn11A lacking the family 36 carbohydrate-binding module) were allowed to interact overnight at room temperature. Lane 1, rS1; lane 2, rXyn11A; lane 3; interaction of rS1 and rXyn11A; lane 4, nS1; lane 5, rXyn11A; lane 6, interaction of nS1 and rXyn11A; lane 7, rXynXL; lane 8, interaction of nS1 and rXynXL. Lanes 1–3 are from the same nondenaturing gel. Lanes 4–6 and lanes 7–8 were cropped from different nondenaturing gels