Fig. 4
From: Identification of novel B cell epitopes in the fiber protein of serotype 8 Fowl adenovirus
Mapping of B cell epitopes recognized by mAbs 5F10 and 4D9. a The different truncated GST-fiber fusion proteins were analyzed using mAbs 5F10 and 4D9 by western blot. The mAb against GST was as positive control. Lane 1, 2, 3 and 4: the lysate of the IPTG induced BL21 cells transformed with pGEX-6p-1, GST-F-1–186aa, GST-F-187–360aa and GST-F-361–519aa, respectively. b The LMH cells transfected with the different truncated fiber genes F-1–186aa, F-1–149aa, F-1–112aa and the control plasmid pcDNA3.1, respectively were analyzed using mAb 5F10 by IFA. c The LMH cells transfected with the different truncated fiber genes F-187–360aa, F-187–287aa, F-187–250aa and the control plasmid pcDNA3.1, respectively were analyzed using mAb 4D9 by IFA. d The different truncated GST-fiber fusion proteins were analyzed using mAbs 5F10 and 4D9 by western blot. The mAb against GST was as positive control. Lane 1, 2, 3 and 4: the lysate of the IPTG induced BL21 cells transformed with pGEX-6p-1, GST-F-113–149aa, pGEX-6p-1 and GST-F-187–219aa, respectively