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Table 1 Bacterial strains and plasmids used in this study

From: Towards the understanding of the enzymatic cleavage of polyisoprene by the dihaem-dioxygenase RoxA

Strain/plasmid Relevant characteristics References
Escherichia coli S17-1 Conjugation strain Simon et al. (1983)
Steroidobacter cummioxidans 35Y Growth on poly(cis-1,4-isoprene) latex, clearing zone formation DSMZ103114
S. cummioxidans 35Y-CM Chloramphenicol resistant mutant of 35Y Hambsch et al. (2010)
S. cummioxidans 35Y-CM ΔroxA-attB (SN3727) Chromosomal deletion of roxA, attB at former roxA site no clearing zone formation on latex agar Birke et al. (2012)
S. cummioxidans 35Y-CM ΔroxA-attB pNH1-roxA-attP (SN4230) Expression of roxA from rhamnose promotor Kmr, Cmr, clearing zone formation in the presence of rhamnose Birke et al. (2012)
S. cummioxidans 35Y-CM ΔroxA(F317Y)-attB pNH1-roxA-attP Expression of roxA (F317Y) from rhamnose promotor, Kmr, Cmr, clearing zone formation in the presence of rhamnose Birke et al. (2012)
S. cummioxidans 35Y-CM ΔroxA(F317L)-attB pNH1-roxA-attP Expression of roxA (F317L) from rhamnose promotor, Kmr, Cmr, clearing zone formation in the presence of rhamnose Birke et al. (2012)
S. cummioxidans 35Y-CM ΔroxA(F317A)-attB pNH1-roxA-attP Expression of roxA (F317A) from rhamnose promotor, Kmr, Cmr, clearing zone formation in the presence of rhamnose Birke et al. (2012)
S. cummioxidans 35Y-CM ΔroxA(F301L)-attB pNH1-roxA-attP Expression of roxA (F317L) from rhamnose promotor, Kmr, Cmr, clearing zone formation in the presence of rhamnose This study
S. cummioxidans 35Y-CM ΔroxA(F301Y)-attB pNH1-roxA-attP Expression of roxA (F317Y) from rhamnose promotor, Kmr, Cmr, clearing zone formation in the presence of rhamnose This study