Table 1 Bacterial strains and plasmids used in this study
From: Towards the understanding of the enzymatic cleavage of polyisoprene by the dihaem-dioxygenase RoxA
Strain/plasmid | Relevant characteristics | References |
|---|---|---|
Escherichia coli S17-1 | Conjugation strain | Simon et al. (1983) |
Steroidobacter cummioxidans 35Y | Growth on poly(cis-1,4-isoprene) latex, clearing zone formation | DSMZ103114 |
S. cummioxidans 35Y-CM | Chloramphenicol resistant mutant of 35Y | Hambsch et al. (2010) |
S. cummioxidans 35Y-CM ΔroxA-attB (SN3727) | Chromosomal deletion of roxA, attB at former roxA site no clearing zone formation on latex agar | Birke et al. (2012) |
S. cummioxidans 35Y-CM ΔroxA-attB pNH1-roxA-attP (SN4230) | Expression of roxA from rhamnose promotor Kmr, Cmr, clearing zone formation in the presence of rhamnose | Birke et al. (2012) |
S. cummioxidans 35Y-CM ΔroxA(F317Y)-attB pNH1-roxA-attP | Expression of roxA (F317Y) from rhamnose promotor, Kmr, Cmr, clearing zone formation in the presence of rhamnose | Birke et al. (2012) |
S. cummioxidans 35Y-CM ΔroxA(F317L)-attB pNH1-roxA-attP | Expression of roxA (F317L) from rhamnose promotor, Kmr, Cmr, clearing zone formation in the presence of rhamnose | Birke et al. (2012) |
S. cummioxidans 35Y-CM ΔroxA(F317A)-attB pNH1-roxA-attP | Expression of roxA (F317A) from rhamnose promotor, Kmr, Cmr, clearing zone formation in the presence of rhamnose | Birke et al. (2012) |
S. cummioxidans 35Y-CM ΔroxA(F301L)-attB pNH1-roxA-attP | Expression of roxA (F317L) from rhamnose promotor, Kmr, Cmr, clearing zone formation in the presence of rhamnose | This study |
S. cummioxidans 35Y-CM ΔroxA(F301Y)-attB pNH1-roxA-attP | Expression of roxA (F317Y) from rhamnose promotor, Kmr, Cmr, clearing zone formation in the presence of rhamnose | This study |