Fig. 2

Immunofluorescence assay and multicellular tumor spheroids permeability detection. a Fluorescent images of hepG2 cells bound by scFab-ANG and scFab, blue represents nuclei stained by DAPI, green represents binding sites of scFab-ANG/scFab staining by FITC. b U87 cells were cultured to form spheroids. c Cy3-labeled scFab-ANG (left) and Cy3-labeled scFab alone (right) were separately added to spheroids and cultured for 20 h, spheroids were imaged using confocal microscopy, red represents region stained by Cy3