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Fig. 2 | AMB Express

Fig. 2

From: Plasticity of the HEK-293 cells, related to the culture media, as platform to produce a subunit vaccine against classical swine fever virus

Fig. 2

Analysis of the E2-CD154 proteins produced in the culture supernatants of HEK-293-E2-CD154 cells growing in the four different commercial media a SDS-PAGE 10% under reducing conditions, b Western blotting using MAb2.3HRP monoclonal antibody 1:5000. MWM: Molecular weight marker; C+: Positive control of E2-CD154 (Suárez et al. 2017); 1: Pro293SCDM medium; 2: CDM4HEK293 medium; 3: SFM4HEK293 medium; 4: CPCHO medium. In all cases, 10 µL of each culture supernatant from HEK-293-E2-CD154 cells were applied. c Productivity of HEK-293-E2-CD154 cells in terms of E2-CD154 protein pg per cell, which was calculated from four replicates in all cases. Error bars indicate the standard deviations. Asterisks mean statistical significance (**p < 0.005) and ***p < 0.0001)

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