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Table 4 Monosaccharide/HEK-G-hPRL molar ratio determination based on N-glycoprofiling

From: Expression of glycosylated human prolactin in HEK293 cells and related N-glycan composition analysis

  Fraction of glycan mass (%) Monosaccharide weight contribution (Da) Mole/G-hPRL mole
Fuc 7.20 119.3 0.82
GlcNAc 50.98 844.4 4.16
Gal 6.71 111.1 0.69
Man 32.77 542.8 3.35
SA 2.55 42.2 0.14
  1. Considering the average N-glycan mass = 1656.3 Da