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Table 2 Molecular mass of HEK cell-derived glycosylated prolactin obtained via N-glycoprofiling analysis and compared to MALDI–TOF-MS determination

From: Expression of glycosylated human prolactin in HEK293 cells and related N-glycan composition analysis

Via N-glycoprofiling

Via MALDI–TOF-MS

Average N-glycan mass (Da)

G-hPRL MM (Da)a

Carbohydrate moiety (%)b

NG-hPRL (Mr)

G-hPRL (Mr)

Carbohydrate moiety (%)

Difference between G-hPRL MM and Mr (%)

1656.3

24,554.1

6.7

23,035.9

25,123.5

8.3

− 2.27

  1. aCalculated by adding the average N-glycan mass to the calculated NG-hPRL mass of 22,897.75 (Capone et al. 2015)
  2. bCalculated as a percent of the average glycan mass on G-hPRL MM
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