Identification and characterization of levulinyl-CoA synthetase from Pseudomonas citronellolis, which differs phylogenetically from LvaE of Pseudomonas putida

AMB Express

Table 1 The ten most up-regulated genes of Pseudomonas citronellolis LA18T with levulinic acid

Scaffold	ORF	Product	Fold change^a
scf_PCLA_07	PCLA_07r0385	Cation/acetate symporter	48.4
scf_PCLA_07	PCLA_07f0384	5-Aminolevulinic acid dehydratase	44.0
scf_PCLA_16	PCLA_16f0148	Putative metalloprotease	41.1
scf_PCLA_05	PCLA_05r0437	Putative structural toxin protein RtxA	30.8
scf_PCLA_05	PCLA_05f0439	TolC family type I secretion outer membrane protein	27.0
scf_PCLA_12	PCLA_12f0276	RNA polymerase sigma factor	21.6
scf_PCLA_15	PCLA_15f0186	Acetyl-CoA carboxylase	21.3
scf_PCLA_02	PCLA_02f0403	Adenylate kinase	18.5
scf_PCLA_01	PCLA_01f0750	NADH dehydrogenase	18.5
scf_PCLA_02	PCLA_02f0217	Putative membrane or periplasmic protein	15.0

^aExpression of genes after 3-day cultivation with LA were compared to that after 2-day cultivation with pyruvic acid (L3/P2)