AMB Express

Table 3 Relative and specific activities of the three immobilized MtFae1a versions on FAE model substrates

From: Glycosylation influences activity, stability and immobilization of the feruloyl esterase 1a from Myceliophthora thermophila

	M-Fae		P-Fae		E-Fae
	Relative activity (%)	Specific activity (µM/min/µg)	Relative activity (%)	Specific activity (µM/min/µg)	Relative activity (%)	Specific activity (µM/min/µg)
Methyl ferulate	70.0 ± 7.3	7.5 ± 0.8	74.4 ± 3.9	3.8 ± 0.2	59.6 ± 19.8	1.3 ± 0.4
Methyl caffeate	85.3 ± 23.4	9.1 ± 2.5	24.2 ± 2.0	1.2 ± 0.1	Trace^a	Trace^a
Methyl sinapate	44.2 ± 6.4	4.7 ± 0.7	19.8 ± 5.3	1.0 ± 0.3	28.3 ± 15.4	0.6 ± 0.3
Methyl p-coumarate	100 ± 20.0	10.7 ± 2.1	100 ± 7.7	5.1 ± 0.4	100 ± 10.5	2.1 ± 0.2

Data were obtained at 35 °C in sodium phosphate at pH 7.5 for M-Fae and E-Fae, and at pH 8.0 for P-Fae, using a stopped assay. The results given are the average of three experiments ± one standard deviation
^aTrace: trace activity observed (less than 0.4 µM/min/µg)

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