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Table 3 Relative and specific activities of the three immobilized MtFae1a versions on FAE model substrates

From: Glycosylation influences activity, stability and immobilization of the feruloyl esterase 1a from Myceliophthora thermophila

  M-Fae P-Fae E-Fae
Relative activity (%) Specific activity (µM/min/µg) Relative activity (%) Specific activity (µM/min/µg) Relative activity (%) Specific activity (µM/min/µg)
Methyl ferulate 70.0 ± 7.3 7.5 ± 0.8 74.4 ± 3.9 3.8 ± 0.2 59.6 ± 19.8 1.3 ± 0.4
Methyl caffeate 85.3 ± 23.4 9.1 ± 2.5 24.2 ± 2.0 1.2 ± 0.1 Tracea Tracea
Methyl sinapate 44.2 ± 6.4 4.7 ± 0.7 19.8 ± 5.3 1.0 ± 0.3 28.3 ± 15.4 0.6 ± 0.3
Methyl p-coumarate 100 ± 20.0 10.7 ± 2.1 100 ± 7.7 5.1 ± 0.4 100 ± 10.5 2.1 ± 0.2
  1. Data were obtained at 35 °C in sodium phosphate at pH 7.5 for M-Fae and E-Fae, and at pH 8.0 for P-Fae, using a stopped assay. The results given are the average of three experiments ± one standard deviation
  2. aTrace: trace activity observed (less than 0.4 µM/min/µg)