Skip to main content
AMB Express
Fig. 2 | AMB Express

Fig. 2

From: Glycosylation influences activity, stability and immobilization of the feruloyl esterase 1a from Myceliophthora thermophila

Fig. 2

SDS-PAGE of the three purified MtFae1a versions before and after deglycosylation treatment with PNGaseF. Purified MtFae1a versions: M-Fae was produced in Myceliophthora thermophila, P-Fae in Pichia pastoris and E-Fae in Escherichia coli. d-M-Fae, d-P-Fae and d-E-Fae are the corresponding samples after deglycosylation treatment with PNGaseF. The molecular weight of PNGaseF ≈ 36 kDa. Migration of the molecular weight ladder is shown in the first lane, and corresponding molecular weights are given on the left. All three enzymes have the same amino acid sequence

Back to article page