Rumen fermentation and microbial community composition influenced by live Enterococcus faecium supplementation

AMB Express

Table 2 Total gas, pH, ammonia–nitrogen, methane, and carbon dioxide concentrations during in vitro rumen fermentation (12 h)

Parameters	Treatments				SEM	P-value
Parameters	Con	T1	T2	T3	SEM	Treatment	Linear	Quadratic
Total gas (mL)	55.15^b	59.45^a	56.36^ab	58.16^ab	0.869	0.017	0.535	0.419
pH	5.43	5.39	5.38	5.38	0.083	0.083	0.037	0.734
Ammonia–nitrogen (mM)	22.28	22.63	20.94	23.16	0.790	0.386	0.271	0.663
Methane (mM/mL)	11.20^a	9.12^b	9.93^b	10.27^ab	0.238	0.001	0.001	0.074
Carbon dioxide (mM/mL)	4.18^ab	3.70^b	4.09^ab	4.30^a	0.145	0.053	0.035	0.555

Con control (no addition), T1 0.1% E. faecium, T2 0.5% E. faecium, T3 1.0% E. faecium, SEM standard error of the mean
Different superscript letters indicate a statistically significant difference. P-value, calculated probability