Cloning of novel bacterial xylanases from lignocellulose-enriched compost metagenomic libraries

AMB Express

Table 1 Details regarding the metagenomic fosmid and plasmid libraries constructed and screened for xylanase activity in this study

Library	FOS-37 °C-CEL	FOS-50 °C-CEL	PLA-50 °C-CEL
Vector	Fosmid—pCC1FOS	Fosmid—pCC1FOS	Plasmid—pCR2.1-TOPO
Number of unique clones	43,000	40,000	760,000
Average insert size	28.5 kb	30.8 kb	5.4 kb
Total library size	1226 Mb	1232 Mb	3900 Mb
No. of xylanase hits	21	18	1
Hit rate	1/58 Mb	1/68 Mb	1/3.9 Gb
Positive clone identifiers	XYL1–21	XYL22–39	XYL40

Number of clones was estimated using serial dilution and plating of the primary library. Average insert size was estimated based on restriction digests of 30–40 individual library clones