Fig. 3

Comparison of gene expression. a Schematic diagram of the cephamycin C biosynthetic gene cluster. b The expression of genes belonging to the cephamycin C biosynthetic gene cluster was examined by real-time quantitative PCR analysis using RNA extracted from Streptomyces clavuligerus strains F613-1, ΔcepRS and ΔcepRScom. Results were normalized against the expression of the 16S rRNA gene and are shown as fold change over the F613-1 control. Data are the mean ± standard deviation of three independent biological experiments. *Compared with F613-1, P < 0.05