Production of β-1,3-glucanase and chitosanase from clostridial strains isolated from the soil subjected to biological disinfestation

AMB Express

Table 4 Products and enzymatic activities in the culture supernatants of strains H110 and TB8 grown with wheat bran and Brassica leaves in the PY broth

Strain	Substrate	Days of cultivation	Products in the culture supernatant (mmol/l)		Activities (Unit/ml)
Strain	Substrate	Days of cultivation	Acetate	Butyrate	β-1,3-Glucanase	Chitosanase
H110	Wheat bran^a	7	1.24 ± 0.05	16.2 ± 0.43	4.92 ± 0.12	1.50 ± 0.08
	Wheat bran	21	2.16 ± 0.31	17.2 ± 0.61	3.70 ± 0.51	1.10 ± 0.19
	Brassica leaf	21	2.51 ± 0.82	7.41 ± 0.12	1.47 ± 0.27	nd
TB8	Wheat bran^a	7	0.99 ± 0.03	16.5 ± 0.45	8.25 ± 0.02	1.32 ± 0.05
	Wheat bran	21	2.10 ± 0.27	15.0 ± 0.38	6.67 ± 0.05	1.72 ± 0.06
	Brassica leaf	21	1.60 ± 0.31	5.94 ± 0.06	1.51 ± 0.47	nd

Products, mean ± SD (n = 2); activities, mean ± SD (n = 4)
Both strains produced small amounts (< 2 mmol/l) of n-butanol
nd not determined
^aCo-incubated with F. oxsporum f. sp. spinaciae strain M2-1. Concentrations of substrates, 1% (w/v)