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Table 4 Products and enzymatic activities in the culture supernatants of strains H110 and TB8 grown with wheat bran and Brassica leaves in the PY broth

From: Production of β-1,3-glucanase and chitosanase from clostridial strains isolated from the soil subjected to biological disinfestation

Strain Substrate Days of cultivation Products in the culture supernatant (mmol/l) Activities (Unit/ml)
Acetate Butyrate β-1,3-Glucanase Chitosanase
H110 Wheat brana 7 1.24 ± 0.05 16.2 ± 0.43 4.92 ± 0.12 1.50 ± 0.08
Wheat bran 21 2.16 ± 0.31 17.2 ± 0.61 3.70 ± 0.51 1.10 ± 0.19
Brassica leaf 21 2.51 ± 0.82 7.41 ± 0.12 1.47 ± 0.27 nd
TB8 Wheat brana 7 0.99 ± 0.03 16.5 ± 0.45 8.25 ± 0.02 1.32 ± 0.05
Wheat bran 21 2.10 ± 0.27 15.0 ± 0.38 6.67 ± 0.05 1.72 ± 0.06
Brassica leaf 21 1.60 ± 0.31 5.94 ± 0.06 1.51 ± 0.47 nd
  1. Products, mean ± SD (n = 2); activities, mean ± SD (n = 4)
  2. Both strains produced small amounts (< 2 mmol/l) of n-butanol
  3. nd not determined
  4. aCo-incubated with F. oxsporum f. sp. spinaciae strain M2-1. Concentrations of substrates, 1% (w/v)