Fig. 4

Relative binding abilities of chimeric nanobodies to CD4 (a) and GFP (b). See also Fig. 1c for details of the illustration. Long block: nanobody scaffolds, small block: CDRs, white block: amino acid sequences derived from GFP-nanobody, black block: amino acid sequences derived from CD4-nanobody. a Relative response of chimeric nanobodies to CD4 compared to the response of CD4-nanobody. See also Additional file 1: Table S4 for the binding level in each experiment before normalization. b Relative response of chimeric nanobodies to GFP compared to the response of GFP-nanobody. See also Additional file 1: Table S5 for the binding level in each experiment before normalization. N = 3, bar = mean ± SD, **p < 0.01, *p < 0.05