Fig. 3
From: Domain swapping of complementarity-determining region in nanobodies produced by Pichia pastoris

Chimeric nanobodies detected in the culture supernatants of P. pastoris transformants. Culture supernatants of transformants (10 μL for each) were loaded. GFP N and CD4 N represents GFP- or CD4-nanobodies, respectively, tagged with long peptide tags containing two FLAG-tags