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AMB Express
Fig. 3 | AMB Express

Fig. 3

From: Immunization with recombinant fusion of LTB and linear epitope (40–62) of epsilon toxin elicits protective immune response against the epsilon toxin of Clostridium perfringens type D

Fig. 3

CHO-K1 cells elongation activity of in vitro assembled holotoxins: ability of the rLTB.Etx40–62 to form functional holotoxin was adjudged by incubating CHO-K1 cells with in vitro assembled holotoxins. CHO-K1 cells (1 × 104 cells/well/100 µl) in a 96 well plate were treated with the reconstituted holotoxins (10 ng/ml) for 16 h at 37 °C in the presence of 1% fetal calf serum under 5% CO2 humidified conditions and visualized under light microscope (×100). a PBS-treated CHO-K1 cells (negative control). b In vitro assembled holotoxin comprising rLTA and wild type rLTB (positive control). c In vitro assembled holotoxin comprising rLTA and rLTB.Etx40–62 fusion protein

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