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Table 4 Box–Behnken design and gossypol content

From: Optimization of the process parameters for reduction of gossypol levels in cottonseed meal by functional recombinant NADPH-cytochrome P450 reductase and cytochrome P450 CYP9A12 of Helicoverpa armigera

Treatment

Factor

Result

A

B

C

D

Y mg/kg

Gossypol content mg/kg

1

33

1.5

2.5

40

65.59

2

37

1.5

2.5

40

58.5

3

33

2.5

2.5

40

46.07

4

37

2.5

2.5

40

45.16

5

35

2

2.25

38

63.21

6

35

2

2.75

38

47.61

7

35

2

2.25

42

67.95

8

35

2

2.75

42

58.11

9

33

2

2.5

38

61.35

10

37

2

2.5

38

50.14

11

33

2

2.5

42

48.12

12

37

2

2.5

42

48.32

13

35

1.5

2.25

40

45.4

14

35

2.5

2.25

40

46.88

15

35

1.5

2.75

40

51.48

16

35

2.5

2.75

40

45.33

17

33

2

2.25

40

56.18

18

37

2

2.25

40

44.56

19

33

2

2.75

40

62.08

20

37

2

2.75

40

53.37

21

35

1.5

2.5

38

73.18

22

35

2.5

2.5

38

42.56

23

35

1.5

2.5

42

48.11

24

35

2.5

2.5

42

51.48

25

35

2

2.5

40

44.11

26

35

2

2.5

40

39.22

27

35

2

2.5

40

36.94

28

35

2

2.5

40

43.87

29

35

2

2.5

40

36.54

  1. This table is the experimental results of Box–Behnken design and corresponding schemes. The gossypol content is selected as the response value Y. The independent variables A, B, C and D was corresponding to initial temperature, enzymatic hydrolysis time, enzyme content, and substrate moisture are shown in the table
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