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Table 2 Box–Behnken experimental factors and coding levels

From: Optimization of the process parameters for reduction of gossypol levels in cottonseed meal by functional recombinant NADPH-cytochrome P450 reductase and cytochrome P450 CYP9A12 of Helicoverpa armigera

Code

Variable

The coding level

− 1

0

1

A

Initial temperature  °C

33

35

37

B

Enzymolysis time h

1.5

2

2.5

C

Enzyme additive amount mL

2.25

2.5

2.75

D

Substrate moisture  %

38

40

42

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