Fig. 1
From: Isolation and identification of a novel protein elicitor from a Bacillus subtilis strain BU412
The purification of the new elicitor protein. a The anion exchange chromatography map. P1-P4 were peaks eluted with a linear gradient of NaCl. b The superdex chromatography map. F1 and F2 were peaks eluted with 20 mM Tris–HCl. Inset, typical chromatogram from a function test of Superdex 75 10/300 GL (Amersham Biosciences). c The SDS-PAGE detection of the target protein. M: low molecular weight standards, 1: the purified protein sample of the peak F2, B1: the target protein band