Table 2 Transformation efficiencies and stability of drug resistance during repeated screenings
Exp. | Plasmid | Total number of Hyg-resistant colonies in the first screening | Number of Hyg-resistant strains/isolates subjected to the repeated screenings | ||
|---|---|---|---|---|---|
2nd | 3rd | 4th | |||
1 | pCsGi-hph | 125 | 6/30 | 0/6 | Not done |
pCsbtubi-hph | 160 | 9/40 | 2/9 | 2/2 | |
pLG-hph | 13 | 0/13 | Not done | Not done | |
2 | pCsGi-hph | 91 | 14/30 | 2/14 | 2/2 |
pCsbtubi-hph | 137 | 11/40 | 4/11 | 4/4 | |
pLG-hph | 20 | 0/20 | Not done | Not done | |
3 | pCsGi-hph | 157 | 23/30 | 7/23 | 7/7 |
pCsbtubi-hph | 170 | 19/40 | 7/19 | 7/7 | |
pLG-hph | 25 | 0/25 | Not done | Not done | |