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Fig. 3 | AMB Express

Fig. 3

From: Stable and transient transformation, and a promoter assay in the selective lignin-degrading fungus, Ceriporiopsis subvermispora

Fig. 3

Detection of the hph cassette in stable transformants treated with pCsGi-hph (a and b) and pCsbtubi-hph (c and d). a, c Southern hybridization analysis. Intact DNA, HindIII- and XhoI-digested total genomic DNA from independent transformants (lanes 1–3, pCsGi-hph; 4–6, pCsbtubi-hph), host wild-type strain (lane W), and the respective plasmid (lane P) were probed with DIG-labeled hph sequence. b, d Specific amplification of hph in genomic PCR. Lane M, DNA marker; Lane W, wild-type host strain as a negative control; Lanes 1–3 and 4–6, pCsGi-hph and pCsbtubi-hph transformants, respectively; Lane P: plasmid pCsGi-hph (b) and pCsbtubi-hph (d) as a positive control

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