Development of high-throughput quantitative analytical method for l-cysteine-containing dipeptides by LC–MS/MS toward its fermentative production

AMB Express

Table 1 Bacterial strains and plasmids used

Strains or plasmids	Genotype	References or sources
Strains
Escherichia coli BW25113	rrnB3 ∆lacZ4787 hsdR514 ∆(araBAD)567 ∆(rhaBAD)568 rph-1	Baba et al. (2006)
Bacillus subtilis 168	trpC2	Takagi et al. (1997)
Plasmids
pACYC184	Tet^R, Cm^R	Nippon Gene
pCys^HP	pACYC184 with serA (T410 Stop), ydeD and alterd cysE (T167A, G203S, T234S, P252L, M256Q) genes under the control of the ompA promoter, Tet^R	Lab strain^a Tanaka et al. (2019)
pQE85	Amp^R, a derivative of pQE80L (Qiagen)	This study
pQE85-BsBacD	pQE85 with bacD gene from Bacillus subtilis	This study

^aThe plasmid pDES (with serA [T410 Stop], ydeD and altered cysE [T167A] genes) from ajinomoto is first supplied (Wiriyathanawudhiwong et al. 2009). However, this plasmid was accidentally obtained in our laboratory during 10 years of stock