Rumen-protected methionine a feed supplement to low dietary protein: effects on microbial population, gases production and fermentation characteristics

AMB Express

Table 7 Effects of experimental treatments on 16S rDNA gene copy numbers of three predominant ruminal and total bacteria from solid fractions using a RUSITEC (n = 4)

Items	Treatments				SEM	P-value
Items	HP	LP	LPLMet	LPHMet	SEM	P-value
Solid fraction, log₁₀ of 16S rDNA gene copy numbers per milliliter solid extracted liquid
Total bacteria	11.79	10.43	10.93	11.90	0.35	0.45
F. succinogenes	8.31	6.99	7.82	8.52	0.27	0.20
R. albus	7.14^a	5.96^b	6.57^ab	7.17^a	0.16	< 0.01
R. flavefaciens	7.38	5.95	6.21	7.43	0.32	0.23
Liquid fraction, log10 of 16S rDNA gene copy numbers per milliliter fermenter liquid
Total bacteria	9.83	8.44	9.00	10.04	0.33	0.30
F. succinogenes	6.23	5.74	5.99	6.73	0.17	0.18
R. albus	6.13^ab	5.12b	5.40^ab	6.21^a	0.17	0.02
R. flavefaciens	5.31	4.40	4.71	5.71	0.28	0.40

SEM, standard error of mean
^a,bSuperscripts values within the same row, are significantly different at (P < 0.05)