Skip to main content

Table 7 Effects of experimental treatments on 16S rDNA gene copy numbers of three predominant ruminal and total bacteria from solid fractions using a RUSITEC (n = 4)

From: Rumen-protected methionine a feed supplement to low dietary protein: effects on microbial population, gases production and fermentation characteristics

Items Treatments SEM P-value
HP LP LPLMet LPHMet
Solid fraction, log10 of 16S rDNA gene copy numbers per milliliter solid extracted liquid
 Total bacteria 11.79 10.43 10.93 11.90 0.35 0.45
 F. succinogenes 8.31 6.99 7.82 8.52 0.27 0.20
 R. albus 7.14a 5.96b 6.57ab 7.17a 0.16 < 0.01
 R. flavefaciens 7.38 5.95 6.21 7.43 0.32 0.23
Liquid fraction, log10 of 16S rDNA gene copy numbers per milliliter fermenter liquid
 Total bacteria 9.83 8.44 9.00 10.04 0.33 0.30
 F. succinogenes 6.23 5.74 5.99 6.73 0.17 0.18
 R. albus 6.13ab 5.12b 5.40ab 6.21a 0.17 0.02
 R. flavefaciens 5.31 4.40 4.71 5.71 0.28 0.40
  1. SEM, standard error of mean
  2. a,bSuperscripts values within the same row, are significantly different at (P < 0.05)