Rumen-protected methionine a feed supplement to low dietary protein: effects on microbial population, gases production and fermentation characteristics

AMB Express

Table 2 Primer sequence used for quantitative real-time PCR analyses

Target bacterial species	Forward primer sequence (5′-3′)	Reverse primer sequence (5′-3′)	Amplicon size (bp)
Total bacteria^a	ACTCCTACGGGAGGCAGCAGT	ATTACCGCGGCTGCTGGC	174
F. succinogenes ^b	GGTATGGGATGAGCTTGC	GCCTGCCCCTGAACTATC	446
R. albus ^b	CCCTAAAAGCAGTCTTAGTTCG	CCTCCTTGCGGTTAGAACA	175
R. flavefaciens ^b	TCTGGAAACGGATGGTA	CCTTTAAGACAGGAGTTTACAA	295

F. succinogenes, Fibrobacter succinogenes; R. albus, Ruminococcus albus; R. flavefaciens, Ruminococcus flavefaciens
^aSchwiertz et al. (2010)
^bKoike and Kobayashi (2001)