Table 4 Summary of purification steps of β-galactosidase from A. niger
Purification steps | Protein (mg/mL) | Activity (UI/mL) | Specific activity (UI/mg) | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|
Crude | 0.150 | 26.000 | 173.333 | 100.000 | 1.000 |
< 100 | 0.144 | 24.000 | 166.667 | 92.308 | 0.962 |
> 30 | 0.065 | 23.440 | 360.615 | 90.154 | 2.080 |
S-200 | 0.0075 | 6.570 | 876.000 | 25.269 | 5.054 |
DEAE | 0.003 | 4.506 | 1502.000 | 17.331 | 8.665 |