Table 1 Strains and plasmids used in this study
From: Isolation and identification of a TetR family protein that regulates the biodesulfurization operon
Strains or plasmid | Relevant features | Source |
|---|---|---|
E. coli DH5α | Cloning strain | Invitrogen |
BL 21(DE3)pLysS | Expression strain | Invitrogen |
Gordonia sp. IITR100 | Completely sequenced, 5.6 Mb | MCC No. 2877 (Singh and Srivastava 2013) |
pET26b | Expression vector, 5.3 kb, KanR, pBR322 ori, T7 promoter | Novagen, USA |
pET29a | Expression vector, 5.3 kb, KanR, pBR322 ori, T7 promoter | Novagen, USA |
pACYC184 | Promoterless plasmid, 4.2 kb, CmR, TetR, p15A ori | |
pRSG43 | KanR,5.2 kb, contains pRC4 replicon | Gifted by Dr. Shavandi (Yamamoto et al. 2011) |
pHYBP109 | AmpR, contains luxAB genes | Gifted by Dr. Victor De Lorenzo |
pTACG | Expression vector,9.2 kb, KanR, tac promoter and dsz operon | This study |
pNG | KanR, 5.6 kb, dsz promoter cloned between NdeI and BglII restriction sites. | This study |
pPM1 | KanR, 5.94 kb, TetR family gene cloned between NdeI and HindIII restriction sites in pET29a vector | This study |
pPM2 | KanR,6 kb, DBRR gene cloned between NdeI and HindIII restriction sites in pET29a vector | This study |
pPM3 | KanR, 5.92 kb, LuxR gene cloned between NdeI and HindIII restriction sites in pET29a vector | This study |
pPM4 | KanR, 5.59 kb, XRE gene cloned between NdeI and HindIII restriction sites in pET29a vector | Murarka and Srivastava (2018) |
pPM6 | KanR, 6.7 kb, Fis gene cloned between NdeI and HindIII restriction sites in pET29a vector | This study |
pPM7 | CmR, 6.68 kb, TetR family gene along with T7 promoter cloned in pACYC184 vector | This study |
pPM8 | KanR, 6.08 kb, TetR family gene cloned between NdeI and HindIII in pTAC vector | This study |
pPM9 | KanR, 8.81 kb, shuttle vector between E. coli and Gordonia/Rhodococcus containing the gene for the TetR family protein | This study |
pTB1 | pET-26b containing3.7 kb dsz operon fragment cloned downstream of 385 bp dsz promoter, KanR | This study |
pTB2 | pTB1 containing pSC101 ori cloned between the sites SspI and EcoRV, SpecR | This study |
pTB3 | pTB1 containing 385 bp dsz promoter fragment cloned between the sites for NdeI and FspI, KanR | This study |
pTB4 | pTB1 containing 315 bp dsz promoter fragment cloned between the sites for NdeI and FspI, KanR | This study |
pTB5 | pTB1 containing 239 bp dsz promoter fragment cloned between the sites for NdeI and FspI, KanR | This study |
pTB6 | pTB1 containing 151 bp dsz promoter fragment cloned between the sites for NdeI and FspI, KanR | This study |
pTB7 | pTB1 containing 63 bp dsz promoter fragment cloned between the sites for NdeI and FspI, KanR | This study |
pPS17 | 11,309 bp, SpecR | Srivastava et al. (2006) |