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Table 1 Strains and plasmids used in this study

From: Isolation and identification of a TetR family protein that regulates the biodesulfurization operon

Strains or plasmid Relevant features Source
E. coli DH5α Cloning strain Invitrogen
BL 21(DE3)pLysS Expression strain Invitrogen
Gordonia sp. IITR100 Completely sequenced, 5.6 Mb MCC No. 2877 (Singh and Srivastava 2013)
pET26b Expression vector, 5.3 kb, KanR, pBR322 ori, T7 promoter Novagen, USA
pET29a Expression vector, 5.3 kb, KanR, pBR322 ori, T7 promoter Novagen, USA
pACYC184 Promoterless plasmid, 4.2 kb, CmR, TetR, p15A ori  
pRSG43 KanR,5.2 kb, contains pRC4 replicon Gifted by Dr. Shavandi (Yamamoto et al. 2011)
pHYBP109 AmpR, contains luxAB genes Gifted by Dr. Victor De Lorenzo
pTACG Expression vector,9.2 kb, KanR, tac promoter and dsz operon This study
pNG KanR, 5.6 kb, dsz promoter cloned between NdeI and BglII restriction sites. This study
pPM1 KanR, 5.94 kb, TetR family gene cloned between NdeI and HindIII restriction sites in pET29a vector This study
pPM2 KanR,6 kb, DBRR gene cloned between NdeI and HindIII restriction sites in pET29a vector This study
pPM3 KanR, 5.92 kb, LuxR gene cloned between NdeI and HindIII restriction sites in pET29a vector This study
pPM4 KanR, 5.59 kb, XRE gene cloned between NdeI and HindIII restriction sites in pET29a vector Murarka and Srivastava (2018)
pPM6 KanR, 6.7 kb, Fis gene cloned between NdeI and HindIII restriction sites in pET29a vector This study
pPM7 CmR, 6.68 kb, TetR family gene along with T7 promoter cloned in pACYC184 vector This study
pPM8 KanR, 6.08 kb, TetR family gene cloned between NdeI and HindIII in pTAC vector This study
pPM9 KanR, 8.81 kb, shuttle vector between E. coli and Gordonia/Rhodococcus containing the gene for the TetR family protein This study
pTB1 pET-26b containing3.7 kb dsz operon fragment cloned downstream of 385 bp dsz promoter, KanR This study
pTB2 pTB1 containing pSC101 ori cloned between the sites SspI and EcoRV, SpecR This study
pTB3 pTB1 containing 385 bp dsz promoter fragment cloned between the sites for NdeI and FspI, KanR This study
pTB4 pTB1 containing 315 bp dsz promoter fragment cloned between the sites for NdeI and FspI, KanR This study
pTB5 pTB1 containing 239 bp dsz promoter fragment cloned between the sites for NdeI and FspI, KanR This study
pTB6 pTB1 containing 151 bp dsz promoter fragment cloned between the sites for NdeI and FspI, KanR This study
pTB7 pTB1 containing 63 bp dsz promoter fragment
cloned between the sites for NdeI and FspI, KanR
This study
pPS17 11,309 bp, SpecR Srivastava et al. (2006)