Fig. 4
From: Cell-free enzymatic synthesis of GDP-l-fucose from mannose
MS (a) and HPLC (b) analysis of the production of GDP-d-mannose by two-step method. Glk was firstly added into the reaction sample and incubation for 10 h, after terminating the reaction by heating, ManB and ManC were simultaneously supplied to the reaction sample for production of GDP-d-mannose. MS (c) and HPLC (d) analysis of the production of GDP-d-mannose by one-step method. Glk, ManB and ManC were simultaneously added into the reaction sample for conversion of mannose to GDP-d-mannose