Comprehensive investigation on the synergistic antibacterial activities of Jatropha curcas pressed cake and seed oil in combination with antibiotics

Haq, Abdul; Siddiqi, Maleeha; Batool, Syeda Zakia; Islam, Arshad; Khan, Alam; Khan, Dildar; Khan, Samiullah; Khan, Haji; Shah, Aamer Ali; Hasan, Fariha; Ahmed, Safia; Badshah, Malik

doi:10.1186/s13568-019-0793-6

AMB Express

Table 7 Minimum inhibitory concentration (MIC) and fractional inhibitory concentration (FIC) of aqueous extract, each antibiotic alone and their combination against MDR pathogenic bacterial isolates

From: Comprehensive investigation on the synergistic antibacterial activities of Jatropha curcas pressed cake and seed oil in combination with antibiotics

Strain	Compound	MIC^a (mg/mL)/MIC^b (µg/mL)		FIC	FICI	Output
Strain	Compound	Alone	Combined	FIC	FICI	Output
MRSA1	Aq/Ctx	–/100	50/25	–/0.25	ND	ND
	Aq/R	–/100	1.56/0.78	–/0.007	ND	ND
	Aq/Of	–/100	100/50	–/0.5	ND	ND
	Aq/Cip	–/50	100/50	–/1	ND	ND
	Aq/Mox	–/1.56	100/50	–/32	ND	ND
MRSA2	Aq/Ctx	–/100	100/50	–/0.5	ND	ND
	Aq/R	–/3.125	0.78/0.39	–/0.1	ND	ND
	Aq/Of	–/25	100/50	–/2	ND	ND
	Aq/Cip	–/50	100/50	–/1	ND	ND
	Aq/Mox	–/0.78	25/12.5	–/16	ND	ND
MRSA3	Aq/Ctx	200/50	100/50	0.5/1	1.5	Indifference
	Aq/R	200/3.13	0.39/0.195	0.001/0.06	0.06	Synergistic
	Aq/Of	200/50	100/50	0.5/1	1.5	Indifference
	Aq/Cip	200/12.5	100/50	2/4	6	Antagonistic
	Aq/Mox	200/0.39	12.5/6.25	0.25/16	16.2	Antagonistic
MRSA4	Aq/Ctx	–/50	100/50	–/1	ND	ND
	Aq/R	–/3.125	100/50	–/16	ND	ND
	Aq/Of	–/1.56	100/50	–/32	ND	ND
	Aq/Cip	–/3.125	100/50	–/16	ND	ND
	Aq/Mox	–/0.78	50/25	–/32	ND	ND
MRSA5	Aq/Ctx	200/3.13	100/50	0.5/16	16.5	Antagonistic
	Aq/R	200/3.13	6.25/3.125	0.03/1	1.03	Indifference
	Aq/Of	200/100	12.5/6.25	0.03/0.06	0.09	Synergistic
	Aq/Cip	200/50	12.5/6.25	0.06/0.1	0.18	Synergistic
	Aq/Mox	200/1.56	6.25/3.125	0.06/2	2.0	Indifference
A. baumannii MDR	Aq/Ctx	–/6.25	100/50	–/8	ND	ND
	Aq/R	–/3.125	0.78/0.39	–/0.1	ND	ND
	Aq/Of	–/0.78	100/50	–/64	ND	ND
	Aq/Cip	–/6.25	100/50	–/8	ND	ND
	Aq/Mox	–/1.56	6.25/3.125	–/2	ND	ND
Klebsiella pneumoniae	Aq/Ctx	–/100	100/50	–/0.5	ND	ND
	Aq/R	–/100	100/50	–/0.5	ND	ND
	Aq/Of	–/25	100/50	–/2	ND	ND
	Aq/Cip	–/50	100/50	–/1	ND	ND
	Aq/Mox	–/1.56	12.5/6.25	–/4	ND	ND
P. chlororaphis	Aq/Ctx	–/–	–/–	–/–	ND	ND
	Aq/R	–/100	100/50	–/0.5	ND	ND
	Aq/Of	–/6.25	50/25	–/4	ND	ND
	Aq/Cip	–/3.125	50/25	–/8	ND	ND
	Aq/Mox	–/1.56	6.25/3.125	–/2	ND	ND
P. monteilii	Aq/Ctx	–/100	100/50	–/0.5	ND	ND
	Aq/R	–/–	1.56/0.78	–/–	ND	ND
	Aq/Of	–/100	1.56/0.78	–/0.007	ND	ND
	Aq/Cip	–/50	0.78/0.39	–/0.007	ND	ND
	Aq/Mox	–/0.78	100/50	–/64	ND	ND

MIC^a minimal inhibitory concentration for aqueous extract when applied alone, MIC^b minimal inhibitory concentration for antibiotic when applied alone, Me methanolic extract, Ctx cefotaxime, R rifampicin, Of ofloxacin, Cip ciprofloxacin, Mox moxifloxacin, ND not determined

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