Fig. 3
From: Expression of benzoyl-CoA metabolism genes in the lignocellulolytic host Caldicellulosiruptor bescii
Relative expression of heterologously expressed benzoate catabolism genes in strain JWCB018 pJOT2 measured by RT-qPCR. Relative expression was calculated with respect to dnaA, a constitutively expressed chromosomal replication initiation protein (Athe_0001). Though all genes appeared to be transcribed, unique peptides were not identified for two proteins: Ferp_1370 (a putative benzoate transporter) and Ferp_1185 (one of four subunits of benzoyl-CoA reductase). *Heterologously expressed Ferroglobus placidus proteins were deemed present if normalized protein abundance was significantly (p value < 0.02, n = 3, log2 fold change > 1) greater in strain JWCB018 pJOT2 than in strain JWCB018 pJGW07. Numbers in parenthesis indicate percentages of each gene’s coding sequence represented by unique peptides identified through intracellular proteomics