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Table 1 Comparison of absolute quantities of DENV1 and GAPDH used for calculation of purification factor

From: A novel method for the capture-based purification of whole viral native RNA genomes

Treatment group Primer <Cq> Copies/µL Total no. of copies Purification factor (fold)
Pre-capture DENV1 10.47 ± 0.08 5.50 × 108 8.25 × 109 5.61 × 102
  GAPDH 14.75 ± 0.1 2.52 × 107 3.78 × 108
Post-capture DENV1 17.14 ± 0.09 1.92 × 107 5.77 × 108
  GAPDH 29.63 ± 0.1 1.57 × 103 4.72 × 104
Pre-capture DENV1 12.13 ± 0.1 2.39 × 108 3.58 × 109 7.91 × 102
  GAPDH 17.04 ± 0.4 5.70 × 106 8.55 × 107
Post-capture DENV1 15.31 ± 0.1 4.83 × 107 1.45 × 109
  GAPDH 29.75 ± 0.07 1.46 × 103 4.37 × 104