Table 1 Comparison of absolute quantities of DENV1 and GAPDH used for calculation of purification factor
From: A novel method for the capture-based purification of whole viral native RNA genomes
Treatment group | Primer | <Cq> | Copies/µL | Total no. of copies | Purification factor (fold) |
|---|---|---|---|---|---|
Pre-capture | DENV1 | 10.47 ± 0.08 | 5.50 × 108 | 8.25 × 109 | 5.61 × 102 |
GAPDH | 14.75 ± 0.1 | 2.52 × 107 | 3.78 × 108 | ||
Post-capture | DENV1 | 17.14 ± 0.09 | 1.92 × 107 | 5.77 × 108 | |
GAPDH | 29.63 ± 0.1 | 1.57 × 103 | 4.72 × 104 | ||
Pre-capture | DENV1 | 12.13 ± 0.1 | 2.39 × 108 | 3.58 × 109 | 7.91 × 102 |
GAPDH | 17.04 ± 0.4 | 5.70 × 106 | 8.55 × 107 | ||
Post-capture | DENV1 | 15.31 ± 0.1 | 4.83 × 107 | 1.45 × 109 | |
GAPDH | 29.75 ± 0.07 | 1.46 × 103 | 4.37 × 104 |