Table 2 Comparison of properties of different C. cinerea laccases
Enzyme properties | Substrate | Lcc1 (Schneider et al. 1999) | Lcc2 (Tian et al. 2014) | Lcc6 (Wang et al. 2014) | Lcc9 (Pan et al. 2014) | Lcc8 this study |
|---|---|---|---|---|---|---|
Molecular weight [kDa] | 66.0 | 54.0 | 57.4 | n. d. | 64 and 77 | |
Optimal pH | ABTS | 4.0 | 2.6 | 3.0 | 2.5 | 4.0 |
DMP | n. d. | n. d. | n. d. | 6.5 | 5.0 | |
guaiacol | n. d. | n. d. | n. d. | 6.5 | 4.5 | |
SGZ | 6.5 | n. d. | n. d. | 6.5 | 5.0 | |
Thermal stability (50 °C) | > 200 min | ca.50 min | > 60 min | n. d. | 300 min | |
Optimal temperature | 60–70 °C | 45 °C | 40 °C | 60 °C | 54–63 °C | |
pH stability (≥ 50%) | 7.0–10.0 4 °C, 14 days | 2.0–11.0 4 °C, 24 h | 2.5–6.0 4 °C, 24 h | 4.5–6.5 37 °C, 8 h | 5.0–11.0 24 °C, 24 h | |
pI value | 3.5 | n. d. | n. d. | n. d. | 3.3/3.4 |