Table 1 Purification of Lcc8 from culture supernatant
Purification step | Total laccase activity [U] | Total protein amount [mg] | Specific activity [U/mg] | Yield [%] | Purification factor |
|---|---|---|---|---|---|
Supernatant | 133 | 28.6 | 4.7 | 100 | 1.0 |
Ultrafiltration | 127 | 22.7 | 5.6 | 95 | 1.2 |
AEX | 85 | 5.4 | 15.7 | 64 | 3.3 |
HIC | 25 | 0.5 | 46.3 | 19 | 9.9 |