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Table 2 Observed, predicted, and residual values for process parameters optimization of l-asparaginase productivity by Bacillus licheniformis mutant using Box–Behnken central composite design

From: Experimental and bioinformatics study for production of l-asparaginase from Bacillus licheniformis: a promising enzyme for medical application

Experiment Temperature (°C) pH Time (h) Agitation (rpm) Square root of l-asparaginase activity (IU/ml/h) Residual value
Observed value Predicted value
1 35 6 33 175 2.52 2.51 0.01
2 40 6 33 175 2.49 2.54 − 0.05
3 35 8 33 175 2.67 2.63 0.04
4 40 8 33 175 2.64 2.65 − 0.01
5 37.5 7 18 150 2.76 2.78 − 0.02
6 37.5 7 48 150 2.45 2.47 − 0.02
7 37.5 7 18 200 2.81 2.79 0.02
8 37.5 7 48 200 2.56 2.55 0.01
9 35 7 33 150 2.66 2.66 0
10 40 7 33 150 2.65 2.62 0.03
11 35 7 33 200 2.61 2.64 − 0.03
12 40 7 33 200 2.74 2.74 0
13 37.5 6 18 175 2.65 2.62 0.03
14 37.5 8 18 175 2.79 2.77 0.02
15 37.5 6 48 175 2.37 2.39 − 0.02
16 37.5 8 48 175 2.46 2.47 − 0.01
17 35 7 18 175 2.76 2.8 − 0.04
18 40 7 18 175 2.8 2.8 0
19 35 7 48 175 2.52 2.5 0.02
20 40 7 48 175 2.6 2.56 0.04
21 37.5 6 33 150 2.53 2.5 0.03
22 37.5 8 33 150 2.57 2.58 − 0.01
23 37.5 6 33 200 2.54 2.52 0.02
24 37.5 8 33 200 2.65 2.66 − 0.01
25* 37.5 7 33 175 2.67 2.68 − 0.01
26* 37.5 7 33 175 2.69 2.68 0.01
27* 37.5 7 33 175 2.68 2.68 0
  1. * The 3 replicates at the center point of the tested values (pH, temperature, time, agitation)