Experimental and bioinformatics study for production of l-asparaginase from Bacillus licheniformis: a promising enzyme for medical application

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Table 2 Observed, predicted, and residual values for process parameters optimization of l-asparaginase productivity by Bacillus licheniformis mutant using Box–Behnken central composite design

Experiment	Temperature (°C)	pH	Time (h)	Agitation (rpm)	Square root of l-asparaginase activity (IU/ml/h)		Residual value
Experiment	Temperature (°C)	pH	Time (h)	Agitation (rpm)	Observed value	Predicted value	Residual value
1	35	6	33	175	2.52	2.51	0.01
2	40	6	33	175	2.49	2.54	− 0.05
3	35	8	33	175	2.67	2.63	0.04
4	40	8	33	175	2.64	2.65	− 0.01
5	37.5	7	18	150	2.76	2.78	− 0.02
6	37.5	7	48	150	2.45	2.47	− 0.02
7	37.5	7	18	200	2.81	2.79	0.02
8	37.5	7	48	200	2.56	2.55	0.01
9	35	7	33	150	2.66	2.66	0
10	40	7	33	150	2.65	2.62	0.03
11	35	7	33	200	2.61	2.64	− 0.03
12	40	7	33	200	2.74	2.74	0
13	37.5	6	18	175	2.65	2.62	0.03
14	37.5	8	18	175	2.79	2.77	0.02
15	37.5	6	48	175	2.37	2.39	− 0.02
16	37.5	8	48	175	2.46	2.47	− 0.01
17	35	7	18	175	2.76	2.8	− 0.04
18	40	7	18	175	2.8	2.8	0
19	35	7	48	175	2.52	2.5	0.02
20	40	7	48	175	2.6	2.56	0.04
21	37.5	6	33	150	2.53	2.5	0.03
22	37.5	8	33	150	2.57	2.58	− 0.01
23	37.5	6	33	200	2.54	2.52	0.02
24	37.5	8	33	200	2.65	2.66	− 0.01
25*	37.5	7	33	175	2.67	2.68	− 0.01
26*	37.5	7	33	175	2.69	2.68	0.01
27*	37.5	7	33	175	2.68	2.68	0

* The 3 replicates at the center point of the tested values (pH, temperature, time, agitation)