Isolation and biochemical characterization of a metagenome-derived 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase gene from subtropical marine mangrove wetland sediments

AMB Express

Table 2 Enzymatic property of representative DAHPSs that were expressed in the E. coli system

Organism	Protein	T	OpH	OT (°C)	K ^PEP_m (µM)	k ^PEP_cat (s⁻¹)	k ^PEP_cat /K ^PEP_m (s⁻¹ µM⁻¹)	References
N. meningitidis	Nme DS	I_α	–	40	11	25	2.3	Cross et al. (2013)
Unculture Microorganisms	Aro1A	I_β	8.0	40	19.58	17.31	0.88	This study
Geobacillus sp.	Gsp DS	I_β	–	–	87	45	0.52	Nazmi et al. (2016)
T. maritima	Tm DS	I_β	6.3	90	9.5	7.6	0.8	Wu et al. (2003)
P. furiosus	Pfu DS	I_β	–	–	120	1.5	0.01	Schofield et al. (2004)
B. subtilis	Bs DS	I_β	9.0	–	139	4.6	0.03	Wu et al. (2005)
P. gingivalis	Pg DS	I_β	9.0	–	421	337	0.80	Wu and Woodard (2006)
A. pernix	Ap DS	I_β	5.7	95	891	1.0	0.001	Zhou et al. (2012)
M. tuberculosis	Mt DS	II	–	–	37	3.1	0.08	Webby et al. (2005a)
C. glutamicum	Cg AroF	II	–	–	160	0.35	0.002	Liu et al. (2008)
C. glutamicum	Cg AroG	II	–	–	8520	1.65	0.0002	Liu et al. (2008)