Synergistic effect of acetyl xylan esterase from Talaromyces leycettanus JCM12802 and xylanase from Neocallimastix patriciarum achieved by introducing carbohydrate-binding module-1

AMB Express

Table 1 Primers used in the study

Prime name	Sequences (5′ → 3′)
axe + cbm1-F	GAATTCGTTGCGGTGGATCACGATG^a
axe + cbm1-R	GCGGCCGCTCACAGACATTGATAATAGTAATCATTGAC^a
axe-F	GAATTCGTTGCGGTGGATCACGATG^a
axe-R	GCGGCCGCTCAAGCAAATCCAAACCATTCC^a
cbm1-F	GGTGAGTCTACTGGTGGCGGAA
cbm1-R	TCACAGACATTGATAATAGTAATCATT
npxyn11-F	GAATTCCAAAGTTTCTGTAGTTCAGCTTCT^a
npxyn11-R	GCGGCCGCTCAATCACCAATGTA^a
npxyn11gene-F	CAAAGTTTCTGTAGTTCAGCTTCT
npxyn11gene-R	ATCACCAATGTAAACCTTTGCGTATG

^aNucleotides incorporated for restriction enzyme digestion are underlined. EcoRΙ: GAATTC; NotΙ: GCGGCCG