Expression in eukaryotic cells and purification of synthetic gene encoding enterocin P: a bacteriocin with broad antimicrobial spectrum

AMB Express

Table 1 Minimum inhibitory concentrations (MICs) of the EntP peptide and various antibiotics

Strain	Antimicrobial activity MIC (ug/ml)
Strain	EntP	Ampicillin	Ceftazidime	Vancomycin	Piperacillin	Gentamicin
Gram positive bacteria
Staphylococcus aureus ATCC 25923	8	≤ 1	≤ 2	≤ 1	ND	≤ 1
Staphylococcus aureus ATCC 29213	8	≤ 1	≤ 2	≤ 1	ND	≤ 1
Enterococcus faecalis ATCC 29212	16	≤ 1	≤ 4	≤ 1	ND	≤ 1
Listeria monocytogenes	32	2	≤ 4	1	4	8
Gram negative bacteria
E. coli ATCC 25922	128	≤ 2	≤ 2	ND	≤ 8	≤ 1
Salmonella typhi	16	2	4	ND	4	≤ 2
Salmonella paratyphi C	ND	2	4	ND	≤ 8	≤ 1
Shigella dysenteriae	128	4	≤ 2	ND	4	4
Pseudomonas aeruginosa ATCC 27853	8	ND	2	ND	≤ 8	≤ 16
Resistant strains
MRSA ATCC 33591	64	ND	16	≥ 16	ND	≥ 16
VRE	64	ND	32	≥ 32	ND	≥ 16
Pseudomonas aeruginosa VIM+	256	ND	≥ 32	ND	≥ 128	≥ 16
Acinetobacter baumannii ATCC 13304	128	ND	≥ 32	ND	≥ 64	≥ 16
Klebsiella pneumonia ATCC 700603	8	> 128	≥ 16	ND	≥ 128	≥ 16

Data are collected as MICs according to the Clinical and Laboratory Standards Institute (CLSI)
ND not determined