Table 1 Total protein and phytase activities in crude culture filtrate, ammonium sulfate precipitate and column elute showing the increase in specific activities with the fold purification of the enzyme produced by A. foetidus MTCC 11682 strain
From: Partial purification and characterization of phytase from Aspergillus foetidus MTCC 11682
Purification steps | Total protein (mg) | Total activity (FTU) | Specific activity (FTU/mg) | Purification (fold) | Yield (%) |
|---|---|---|---|---|---|
Crude culture filtrate | 3600 | 45400 | 12.6 | 1 | 100 |
Ammonium sulfate ppt. | 245 | 10200 | 41.6 | 3.3 | 22.5 |
Sephacryl S-200HR | 20 | 5879 | 294.5 | 23.4 | 12.9 |