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AMB Express
Fig. 1 | AMB Express

Fig. 1

From: Characterization, expression and application of a zearalenone degrading enzyme from Neurospora crassa

Fig. 1

a Electrophoresis analysis of expression plasmid. lane M: marker; lane 1: recombined plasmid; lane 2: recombined plasmid was digested by EcoRI and NotI; lane 3: pPIC9K was digested by EcoRI and NotI. b Electrophoresis analysis of PCR. lane M: marker; lane 1: PCR of P. pastoris; lane 2: PCR of recombinant P. pastoris. c Alignment of the deduced amino acid sequence of ZENC (1, upper line) with 2-(acetamidomethylene) succinate hydrolase from Mesorhizobium japonicum (2, lower line). Identical (.) are shown. Residues that comprise the catalytic triad of a serine hydrolase family are boxed

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