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Table 1 Summary of the purification procedure for the recombinant choA

From: Expression optimization of recombinant cholesterol oxidase in Escherichia coli and its purification and characterization

Steps

Total activitya (U)

Total protein (mg)

Specific activity (U/mg)

Purification (fold)

Yield (%)

Crude extractb

78.5

63

1.25

1

100

Ni-CAM affinity chromatography

67.7

9.57

7.07

5.66

86.2

  1. aCholesterol oxidation activity was assayed by measuring H2O2 generation
  2. bCrude extract was obtained from 500 mL of the culture of BL21(DE3)pLysS-pET24-ChoA
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