Fig. 6From: Expression optimization of recombinant cholesterol oxidase in Escherichia coli and its purification and characterizationSDS-PAGE analysis of recombinant ChOA purification steps. Lane 1: crude extract, lane 2: column flow-through, lane 3: protein marker, lane 4: 50 mM imidazole elution, lanes 5–9: 200 mM imidazole elutionBack to article page