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Fig. 2 | AMB Express

Fig. 2

From: Transgenic Nicotiana tabacum seeds expressing the Mycobacterium tuberculosis Alanine- and Proline-rich antigen

Fig. 2

Detection of the APA gene in transgenic tobacco plants. Genomic DNA was amplified with specific primers to detect an 871 bp fragment (indicated by an arrow) corresponding to the APA gene. a Lane 1: negative control; Lanes 2 and 3: transgenic plants; Lane 4: DNA size marker; Lane 5: positive control. b Lane 1: negative control; Lanes 2–5: transgenic plants; Lane 6: positive control; Lane 7: DNA size marker. The negative control was the amplification of the genomic DNA from wild-type plants; the positive control was the amplification from pCam-PSC-APA plasmid DNA and the DNA size marker used was the Gene Ruler 1 kB (Fermentas, USA)

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