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Fig. 4 | AMB Express

Fig. 4

From: Light-harvesting protein Lhcx3 is essential for high light acclimation of Phaeodactylum tricornutum

Fig. 4

Molecular characteristics of transgenic lines. a Ble gene in overexpressed lines detected by genomic PCR; Lane M: 5000 bp marker, Lane 1, 2: A 1 kb band corresponding to Ble gene; Lane 3: genomic DNA of WT cells; Lane 4: water; b Ble gene in knocked-down lines detected by genomic PCR; Lane M: 5000 bp marker, Lane 1, 2: A 1 kb band corresponding to Ble gene; Lane 3: genomic DNA of WT cells; Lane 4: water; c Relative transcript level of Lhcx3 in overexpressed lines (OE1 and OE2) and wild type. Significant difference between WT and transgenic lines is indicated at P < 0.05 (*) or P < 0.01 (**) level. Each value represents mean ± SD (n = 3); d Relative transcript level of Lhcx3 in knocked-down lines (KNO1 and KNO2) and wild type. LL = 30 μmol m−2 s−1, HL = 800 μmol m−2 s−1

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