Fig. 1
From: Light-harvesting protein Lhcx3 is essential for high light acclimation of Phaeodactylum tricornutum
Amino acid sequence analysis of PtLhcx3 and design of PtLhcx3 expression construct. a The conserved domain of PtLhcx3 amino acid sequence in P. tricornutum. The amino acid sequences of Lhcx1 (Phatr 27278) and Lhcx2 (Phatr 54065) from P. tricornutum, Lhcx2 (Thapsd_38879) and Lhcx LI818 (Thapsd 264921) from T. pseudonana, and LI818 (Chlre 184724) from Chlamydomonas reinhardtii were shown. b Phylogenetic tree showing evolutionary position of PtLhcx3. The top 25 hit were used as reference sequences when amino acid sequence of PtLhcx3 was searched against non-redundant protein database by BLASTP. Phylogenetic tree was constructed using the Neighbor-Joining method in MEGA7. The phylogenetic tree was tested by bootstrapping with 500 replicates and the percentages of bootstrap support were given on the branches. The PtLhcx3 was indicated by the blue arrow. c The schematic map showing cassettes of PtLhcx3 overexpression and knockdown driven by PfcpC promoter. An omega-leader was used for the enhancement of transcription