Fig. 5

Sequence alignment of AN1602 and AA9-LPMOs with known structures. The proteins included (PDB ID in parenthesis) are: Ls(AA9)A (5ACH), NcLPMO9C (4D7U), NcLPMO9D (4EIR), NcLPMO9M (4EIS), TaGH61A (2YET), Cel61B (2VTC), PcGH61D (4B5Q) TtGH61E (3EII) and NcLPMO9F (4QI8). Conserved residues Asn30, His69 and His70 in AN1602 and their equivalent residues in Ls(AA9)A and NcLPMO9C that potentially determine the activity on cello-oligomers are highlighted in green. Conserved His1 is shown in turquoise. Cluster of three consecutive aspargine residues in NcLPMO9C and Ls(AA9)A is boxed. Residues corresponding to Insertion loop L3 is shown as a horizontal bar below the sequence